Quantification of intracellular HNO delivery with capillary zone electrophoresis.
Nitric Oxide
; 118: 49-58, 2022 01 01.
Article
em En
| MEDLINE
| ID: mdl-34715361
ABSTRACT
Redox signaling, wherein reactive and diffusible small molecules are channeled into specific messenger functions, is a critical component of signal transduction. A central principle of redox signaling is that the redox modulators are produced in a highly controlled fashion to specifically modify biotargets. Thiols serve as primary mediators of redox signaling as a function of the rich variety of adducts, which allows initiation of distinct cellular effects. Coupling the inherent reactivity of thiols with highly sensitive and selective chemical analysis protocols can facilitate identification of redox signaling agents, both in solution and in cultured cells. Here, we describe use of capillary zone electrophoresis to both identify and quantify sulfinamides, which are specific markers of the reaction of thiols with nitroxyl (HNO), a putative biologically relevant reactive nitrogen species.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óxidos de Nitrogênio
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article