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Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures.
Bach, Katharina; Edel, Birgit; Höring, Steffen; Bartonickova, Lucie; Glöckner, Stefan; Löffler, Bettina; Bahrs, Christina; Rödel, Jürgen.
Afiliação
  • Bach K; Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Edel B; Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Höring S; Division of Infection Control and Infectious Diseases, RWTH Aachen University Hospital, Aachen, Germany.
  • Bartonickova L; Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Glöckner S; Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Löffler B; Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Bahrs C; Institute for Infectious Diseases and Infection Control, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Rödel J; Division of Infectious Diseases and Tropical Medicine, Department of Medicine I, Medical University of Vienna, Vienna, Austria.
Eur J Clin Microbiol Infect Dis ; 41(3): 489-494, 2022 Mar.
Article em En | MEDLINE | ID: mdl-34807364
The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for blaCTX-M, 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacteriemia / Infecções por Escherichia coli Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacteriemia / Infecções por Escherichia coli Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article