Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.
STAR Protoc
; 2(4): 100913, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34917972
ABSTRACT
The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive. For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ductos Pancreáticos
/
Técnicas de Cultura de Células
/
Células-Tronco Pluripotentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article