Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum.
Methods Mol Biol
; 2424: 167-175, 2022.
Article
em En
| MEDLINE
| ID: mdl-34918294
ABSTRACT
The omentum is the metastatic site for intra-abdominal cancers such as colon, stomach, and ovarian (where it is the primary site for metastasis). Adipocytes are the primary cell type of the omentum, and they aid in cancer cell proliferation, migration, and invasion. Therefore, systematic characterization of adipocyte-cancer cell interactions will help in understanding the metastatic spread of intra-abdominal cancer. Here, a detailed mechanical-enzymatic digestion method describes the isolation of both normal and cancer-associated adipocyte from omental tissues.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Omento
/
Neoplasias Ovarianas
Tipo de estudo:
Risk_factors_studies
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article