Analysis of Autophagic Vesicles in Mitotic Cells.
Methods Mol Biol
; 2445: 127-137, 2022.
Article
em En
| MEDLINE
| ID: mdl-34972990
ABSTRACT
The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging of dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution.
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1
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Mitose
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article