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A protocol to inject ocular drug implants into mouse eyes.
Lin, Cheng-Hui; Sun, Young Joo; Lee, Soo Hyeon; Mujica, Elena M; Kunchur, Caitlin R; Wu, Man-Ru; Yang, Jing; Jung, Youn Soo; Chiang, Bryce; Wang, Sui; Mahajan, Vinit B.
Afiliação
  • Lin CH; Byers Eye Institute, Department of Ophthalmology, Stanford University, Stanford, CA 94304, USA.
  • Sun YJ; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Lee SH; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Mujica EM; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Kunchur CR; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Wu MR; Byers Eye Institute, Department of Ophthalmology, Stanford University, Stanford, CA 94304, USA.
  • Yang J; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Jung YS; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
  • Chiang B; Byers Eye Institute, Department of Ophthalmology, Stanford University, Stanford, CA 94304, USA.
  • Wang S; Byers Eye Institute, Department of Ophthalmology, Stanford University, Stanford, CA 94304, USA.
  • Mahajan VB; Molecular Surgery Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94304, USA.
STAR Protoc ; 3(1): 101143, 2022 03 18.
Article em En | MEDLINE | ID: mdl-35141566
ABSTRACT
Ocular drug implants (ODIs) are beneficial for treating ocular diseases. However, the lack of a robust injection approach for small-eyed model organisms has been a major technical limitation in developing ODIs. Here, we present a cost-effective, minimally invasive protocol to deliver ODIs into the mouse vitreous called Mouse Implant Intravitreal Injection (MI3). MI3 provides two alternative surgical approaches (air-pressure or plunger) to deliver micro-scaled ODIs into milli-scaled eyes, and expands the preclinical platforms to determine ODIs' efficacy, toxicity, and pharmacokinetics. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Corpo Vítreo Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Corpo Vítreo Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article