BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.
Nat Commun
; 13(1): 1658, 2022 03 29.
Article
em En
| MEDLINE
| ID: mdl-35351876
ABSTRACT
The process of epigenetic silencing, while fundamentally important, is not yet completely understood. Here we report a replenishable female mouse embryonic stem cell (mESC) system, Xmas, that allows rapid assessment of X chromosome inactivation (XCI), the epigenetic silencing mechanism of one of the two X chromosomes that enables dosage compensation in female mammals. Through a targeted genetic screen in differentiating Xmas mESCs, we reveal that the BAF complex is required to create nucleosome-depleted regions at promoters on the inactive X chromosome during the earliest stages of establishment of XCI. Without this action gene silencing fails. Xmas mESCs provide a tractable model for screen-based approaches that enable the discovery of unknown facets of the female-specific process of XCI and epigenetic silencing more broadly.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Inativação do Cromossomo X
/
RNA Longo não Codificante
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article