Aeromicrobium stalagmiti sp. nov., isolated from a lava cave.
Int J Syst Evol Microbiol
; 72(6)2022 Jun.
Article
em En
| MEDLINE
| ID: mdl-35675105
ABSTRACT
A Gram-reaction-positive, strictly aerobic, non-sporulating, non-motile, rod-shaped bacterium, designated YC3-14T, was isolated from pieces of stalagmite collected in a lava cave in Jeju, Republic of Korea. Cells showed growth at 15-35 °C, pH 6.0-9.0 and with 0-3â% (w/v) NaCl. Colonies of the cells were circular, smooth, convex and cream in colour. A 16S rRNA gene-based neighbour-joining tree indicated that the organism belonged to the genus Aeromicrobium and formed a sublineage between an Aeromicrobium endophyticum-Aeromicrobium fastidiosum cluster and an Aeromicrobium yanjiei-Aeromicrobium chenweiae cluster. The highest 16S rRNA gene similarity values of strain YC3-14T were with the type strains of A. yanjiei (99.2â%), A. endophyticum (99.1â%), A. fastidiosum (98.8â%), A. ginsengisoli (98.8â%) and A. chenweiae (98.7â%). The cell-wall peptidoglycan contained ll-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-9(H4). The predominant fatty acids were C18ââ0.10-methyl, C18ââ1 ω9c and C16ââ0. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and two unidentified lipids. The G+C content of the genome DNA was 69.9âmol%. These chemotaxonomic features of the isolate were typical for the genus Aeromicrobium. The genome-based phylogeny showed the same tree topology as the 16S rRNA gene phylogeny. The average nucleotide identity (≤84.5â%) and digital DNA-DNA hybridization (≤27.5â%) values supported that the isolate belongs to a novel species of the genus Aeromicrobium. On the basis of data obtained by a polyphasic approach, strain YC3-14T (=KCTC 49469T=NBRC 114653T) represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium stalagmiti sp. nov. is proposed.
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MEDLINE
Assunto principal:
Actinomycetales
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Ácidos Graxos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article