Tyrosinase-Mediated Synthesis of Nanobody-Cell Conjugates.
ACS Cent Sci
; 8(7): 955-962, 2022 Jul 27.
Article
em En
| MEDLINE
| ID: mdl-35912347
ABSTRACT
A convenient enzymatic strategy is reported for the modification of cell surfaces. Using a tyrosinase enzyme isolated from Agaricus bisporus, unique tyrosine residues introduced at the C-termini of nanobodies can be site-selectively oxidized to reactive o-quinones. These reactive intermediates undergo rapid modification with nucleophilic thiol, amine, and imidazole residues present on cell surfaces, producing novel nanobody-cell conjugates that display targeted antigen binding. We extend this approach toward the synthesis of nanobody-NK cell conjugates for targeted immunotherapy applications. The resulting NK cell conjugates exhibit targeted cell binding and elicit targeted cell death.
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MEDLINE
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En
Ano de publicação:
2022
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Article