[Identification of metabolites of imperatorin in rats: based on UHPLC-Q-Exactive Orbitrap MS].
Zhongguo Zhong Yao Za Zhi
; 47(15): 4202-4213, 2022 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-36046911
ABSTRACT
This study aims to identify and analyze the metabolites of imperatorin in rats by UHPLC-Q-Exactive Orbitrap MS. Specifically, after rats were treated(ig) with imperatorin, the plasma, urine, and feces were collected, and the samples were processed by solid phase extraction. Then, UHPLC-Q-Exactive Orbitrap MS was performed. In MS, 0.1% formic acid water(A)-acetonitrile(B) was applied as mobile phase for gradient elution and the data of MS in both positive and negative ion modes were collected. The metabolites of imperatorin in blood, urine, and feces of rats were analyzed to explore the metabolic pathways of imperatorin in rats. According to accurate molecular weight, multistage MS data, MS fragmentation rule of the standard substance, and previous reports, a total of 51 metabolites were identified, with 35, 40, and 16 from plasma, urine, and feces, separately. The main metabolic pathways were oxidization, glucuronidation, isopentenyl removal, sulphation, carboxylation, among others. The conclusion in this study is expected to serve as a reference for the further development and the further pharmacodynamics study of imperatorin.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Extração em Fase Sólida
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article