Molecular recognition of methylated amino acids and peptides by Pillar[6]MaxQ.
Org Biomol Chem
; 20(37): 7429-7438, 2022 09 28.
Article
em En
| MEDLINE
| ID: mdl-36097881
ABSTRACT
We report the molecular recognition properties of Pillar[n]MaxQ (P[n]MQ) toward a series of (methylated) amino acids, amino acid amides, and post-translationally modified peptides by a combination of 1H NMR, isothermal titration calorimetry, indicator displacement assays, and molecular dynamics simulations. We find that P6MQ is a potent receptor for N-methylated amino acid side chains. P6MQ recognized the H3K4Me3 peptide with Kd = 16 nM in phosphate buffered saline.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Aminoácidos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article