RNAi-mediated silencing of an egg-specific gene Nllet1 results in hatch failure in the brown planthopper.

ABSTRACT

BACKGROUND: The brown planthopper (BPH) is one of the most destructive agricultural pests in Asia. RNA interference (RNAi)-mediated pest management has been under development for years, and the selection of appropriate target genes is important for pest-targeted RNAi. C-type lectins (CTLs) are a class of genes that perform a variety of functions, such as the regulation of growth and development. RESULTS: A CTL-S protein named Nllet1, containing a single calcium ion (Ca2+ )-dependent carbohydrate-binding domain (CRD) with a conserved triplet motif QPD was identified and functionally characterized in BPH. Expression profiles at both the transcriptional and translational levels show that Nllet1 accumulates during the serosal cuticle (SC) formation period. Immunofluorescence and immunogold labeling further demonstrated that Nllet1 is located in the serosal endocuticle (en-SC). Maternal RNAi-mediated silencing of Nllet1 disrupted the SC structure, accompanied by a loss of the outward barrier and 100% embryo mortality. Injection of 10 ng dsNllet1 or dsNllet1' per female adult BPH resulted in a total failure of egg hatching. CONCLUSION: Nllet1 is essential for SC formation and embryonic development in BPH, which helps us understand the important roles of CTL-Ss. Additionally, BPH eggs show high sensitivity to the depletion of Nllet1. This study indicates that Nllet1 is a promising candidate gene that can be used to develop RNAi-based control strategies at the BPH egg stage, and it can also be used as a target for developing novel ovicides. © 2022 Society of Chemical Industry.