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Slice & Dice: nested spin-lattice relaxation measurements.
Franks, W Trent; Tognetti, Jacqueline; Lewandowski, Józef R.
Afiliação
  • Franks WT; Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. J.R.Lewandowski@warwick.ac.uk.
  • Tognetti J; Department of Physics, University of Warwick, Coventry CV4 7AL, UK.
  • Lewandowski JR; Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. J.R.Lewandowski@warwick.ac.uk.
Phys Chem Chem Phys ; 25(8): 6044-6049, 2023 Feb 22.
Article em En | MEDLINE | ID: mdl-36281524
ABSTRACT
Spin-lattice relaxation rate (R1) measurements are commonly used to characterize protein dynamics. However, the time needed to collect the data can be quite long due to long relaxation times of the low-gamma nuclei, especially in the solid state. We present a method to collect backbone heavy atom relaxation data by nesting the collection of datasets in the solid state. This method results in a factor of 2 to 2.5 times faster data acquisition for backbone R1 relaxation data for the 13C and 15N sites of proteins.

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article