Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease.
Curr Res Food Sci
; 5: 2135-2145, 2022.
Article
em En
| MEDLINE
| ID: mdl-36387593
ABSTRACT
The present study aimed to determine the effects of polysaccharides-riched Prunus mume fruit juice concentrate (PFC) on uric acid (UA) excretion and the gut microbiota in mice with chronic kidney disease (CKD). C57BL/6 mice were randomly allocated to four groups two that were fed AIN93M diet, one of which was administered 500 mg/kg PFC, and two that were fed AIN93M diet containing 0.2% adenine, one of which was administered 500 mg/kg PFC. PFC promoted UA excretion, which may have been mediated through increases in the protein expression of ATP-binding cassette transporter G2 (ABCG2), organic anion transporter 1 (OAT1), organic carnitine transporter 2 (OCTN2), and reductions in the protein expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in kidneys of CKD mice. ABCG2 expression in the intestine was also increased by PFC administration. Additionally, PFC significantly increased large intestinal short-chain fatty acids (SCFAs) concentrations, and the number of gut microbial species, and reduced the abundance of the genera Bacteroides, Pseudoflavonifractor, Helicobacter, Clostridium_IV and Allobaculum, which have a negative effect on UA excretion. In conclusion, PFC may promote UA excretion in CKD mice by altering the expression of urate transporters and regulating the gut microbiota.
ABCG2, ATP-binding cassette transporter G2; BUN, blood urea nitrogen; CKD, chronic kidney disease; Chronic kidney disease; GAE, gallic acid equivalent; GLUT9, glucose transporter 9; Gut microbiota; HPIC, high-performance ion chromatography; HPLC, high-performance liquid chromatography; Lefse, linear discriminant analysis effect size; OAT1, organic anion tranporter 1; OCTN2, organic carnitine transporter 2; OTU, operational taxonomic unit; PCoA, principal components analysis; PFC, Prunus mume fruit juice concentrate; PVDF, polyvinylidene fluoride; Prunus mume; SCFAs, short-chain fatty acids; Short-chain fatty acids; UA, uric acid; URAT1, urate transporter 1; Urate transporters
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article