In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons.
STAR Protoc
; 4(1): 101963, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36542520
ABSTRACT
In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Colículos Superiores
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article