CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion.

Yu, Xinyu; Li, Shuang; Feng, Huibao; Liao, Xihao; Xing, Xin-Hui; Bai, Zhonghu; Liu, Xiuxia; Zhang, Chong

Yu, Xinyu; Li, Shuang; Feng, Huibao; Liao, Xihao; Xing, Xin-Hui; Bai, Zhonghu; Liu, Xiuxia; Zhang, Chong.

Afiliação

Yu X; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Li S; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Feng H; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Liao X; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Xing XH; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China; Institute of Biopharmaceutical and Health Engi
Bai Z; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Jiangsu Provincial Research Center f
Liu X; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Jiangsu Provincial Research Center f
Zhang C; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China. Electronic address: chongzhang@tsinghua.edu.cn

Metab Eng ; 75: 192-204, 2023 01.

Article em En | MEDLINE | ID: mdl-36572334

Assuntos

Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas; Microfluídica; Proteínas Recombinantes/genética; Sistemas CRISPR-Cas/genética

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microfluídica / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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