Dosage differences in 12-OXOPHYTODIENOATE REDUCTASE genes modulate wheat root growth.
Nat Commun
; 14(1): 539, 2023 02 01.
Article
em En
| MEDLINE
| ID: mdl-36725858
ABSTRACT
Wheat, an essential crop for global food security, is well adapted to a wide variety of soils. However, the gene networks shaping different root architectures remain poorly understood. We report here that dosage differences in a cluster of monocot-specific 12-OXOPHYTODIENOATE REDUCTASE genes from subfamily III (OPRIII) modulate key differences in wheat root architecture, which are associated with grain yield under water-limited conditions. Wheat plants with loss-of-function mutations in OPRIII show longer seminal roots, whereas increased OPRIII dosage or transgenic over-expression result in reduced seminal root growth, precocious development of lateral roots and increased jasmonic acid (JA and JA-Ile). Pharmacological inhibition of JA-biosynthesis abolishes root length differences, consistent with a JA-mediated mechanism. Transcriptome analyses of transgenic and wild-type lines show significant enriched JA-biosynthetic and reactive oxygen species (ROS) pathways, which parallel changes in ROS distribution. OPRIII genes provide a useful entry point to engineer root architecture in wheat and other cereals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raízes de Plantas
/
Oxirredutases atuantes sobre Doadores de Grupo CH-CH
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article