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Upregulation of miRNA-10a-5p promotes tumor progression in cervical cancer by suppressing UBE2I signaling.
Gu, Yannan; Feng, Xiaodan; Jin, Yanqi; Liu, Yuanlin; Zeng, Li; Zhou, Dachun; Feng, Yuling.
Afiliação
  • Gu Y; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Feng X; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Jin Y; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Liu Y; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Zeng L; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Zhou D; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
  • Feng Y; Department of Obstetrics and Gynecology, Affiliated Maternal and Child Health Hospital of Nantong University, Nantong, China.
J Obstet Gynaecol ; 43(1): 2171283, 2023 Dec.
Article em En | MEDLINE | ID: mdl-36744815
ABSTRACT
Cervical cancer (CC) is a common malignant neoplasm in gynecology. There is increasing evidence to suggest that microRNAs (miRNAs) act as crucial regulators of CC. However, whether miR-10a-5p plays a role in CC is under investigation. The aim of this stuy was to assess the miR-10a-5p expression pattern in the development of CC and investigate its downstream target. MiR-10a-5p inhibition decreased CC cell proliferation and impaired CC cell invasion and migration but enhanced apoptosis. UBE2I was a direct target of miR-10a-5p. QRT-PCR results showed a down-regulation of UBE2I in CC cells, opposing miR-10a-5p. Besides, overexpression of miR-10a-5p down-regulated UBE2I. Functional rescue experiments further indicated the miR-10a-5p-UBE2I axis was linked to CC cell growth, apoptosis and metastasis. MiR-10a-5p upregulation promotes cervical cancer development by inhibiting UBE2I. These results also predict that miR-10a-5p may be a potential target for the clinical treatment of CC.IMPACT STATEMENTWhat is already known on this subject? As a widely researched cancer-related miRNA, the overexpression of miR-10a-5p has been verified in various cancers. It has been described in a meta-analysis report that there were 42 miRNAs up-regulated and 21 miRNAs down-regulated in different stages of cervical cancer tissue versus healthy tissue.What do the results of this study add? We verified that miR-10a-5p initiates and promotes tumor cell development by decreasing UBE2I abundance. This miR-10a-5p-mediated post-transcriptional regulation of UBE2I is involved in the tumorigenesis, invasion and migration of human cervical cancer.What are the implications of these findings for clinical practice and/or further research? These findings provide mechanistic insights into how miR-10a-5p regulates cervical cancer hyper-proliferation and metastasis, as well as a new target for clinical treatment. Nevertheless, whether miR-10a-5p/UBE2I axis can be regulated by non-invasive methods need further exploration, which will be the focus of our future research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / MicroRNAs / Enzimas de Conjugação de Ubiquitina Tipo de estudo: Prognostic_studies / Systematic_reviews Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / MicroRNAs / Enzimas de Conjugação de Ubiquitina Tipo de estudo: Prognostic_studies / Systematic_reviews Limite: Female / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article