U1 snRNP increases RNA Pol II elongation rate to enable synthesis of long genes.
Mol Cell
; 83(8): 1264-1279.e10, 2023 04 20.
Article
em En
| MEDLINE
| ID: mdl-36965480
ABSTRACT
The expansion of introns within mammalian genomes poses a challenge for the production of full-length messenger RNAs (mRNAs), with increasing evidence that these long AT-rich sequences present obstacles to transcription. Here, we investigate RNA polymerase II (RNAPII) elongation at high resolution in mammalian cells and demonstrate that RNAPII transcribes faster across introns. Moreover, we find that this acceleration requires the association of U1 snRNP (U1) with the elongation complex at 5' splice sites. The role of U1 to stimulate elongation rate through introns reduces the frequency of both premature termination and transcriptional arrest, thereby dramatically increasing RNA production. We further show that changes in RNAPII elongation rate due to AT content and U1 binding explain previous reports of pausing or termination at splice junctions and the edge of CpG islands. We propose that U1-mediated acceleration of elongation has evolved to mitigate the risks that long AT-rich introns pose to transcript completion.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Polimerase II
/
Ribonucleoproteína Nuclear Pequena U1
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article