Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification.

Manojlovic, Zarko; Wlodarczyk, Jordan; Okitsu, Cindy; Jin, Yuxin; Van Den Berg, David; Lieber, Michael R; Hsieh, Chih-Lin

Manojlovic, Zarko; Wlodarczyk, Jordan; Okitsu, Cindy; Jin, Yuxin; Van Den Berg, David; Lieber, Michael R; Hsieh, Chih-Lin.

Afiliação

Manojlovic Z; Department of Urology, University of Southern California, 1441 Eastlake Ave., NTT5420, Los Angeles, CA, USA.
Wlodarczyk J; Department of Surgery, University of Southern California, Los Angeles, CA, USA.
Okitsu C; Department of Pathology, University of Southern California, Los Angeles, CA, USA.
Jin Y; Department of Translational Genomics, Keck School of Medicine of USC, Los Angeles, CA, USA.
Van Den Berg D; Department of Population and Public Health Sciences, University of Southern California, Los Angeles, CA, USA.
Lieber MR; Department of Pathology, University of Southern California, Los Angeles, CA, USA.
Hsieh CL; Department of Urology, University of Southern California, 1441 Eastlake Ave., NTT5420, Los Angeles, CA, USA. hsieh_c@med.usc.edu.

BMC Res Notes ; 16(1): 66, 2023 Apr 27.

Article em En | MEDLINE | ID: mdl-37106434

Assuntos

DNA; Técnicas de Amplificação de Ácido Nucleico; Humanos; Sequenciamento Completo do Genoma; Análise de Sequência de DNA/métodos; Reação em Cadeia da Polimerase; Sequenciamento de Nucleotídeos em Larga Escala/métodos

Palavras-chave

High coverage whole-genome sequencing; Next generation sequencing; Sequencing library construction without DNA extraction

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Técnicas de Amplificação de Ácido Nucleico Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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