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The activity of methylene blue against asexual and sexual stages of Plasmodium vivax.
Fabbri, Camila; Quaresma Ramos, Glenda; Clarys Baia-da-Silva, Djane; Oliveira Trindade, Alexandre; Carlos Salazar-Alvarez, Luis; Costa Ferreira Neves, Juliana; Dos Santos Bastos, Ivanildes; Guimarães Costa, Allyson; Vinicius Guimarães Lacerda, Marcus; Marcelo Monteiro, Wuelton; Trindade Maranhão Costa, Fabio; Costa Pinto Lopes, Stefanie.
Afiliação
  • Fabbri C; Instituto Leônidas & Maria Deane, Fiocruz Amazônia, Manaus, Brazil.
  • Quaresma Ramos G; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil.
  • Clarys Baia-da-Silva D; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil.
  • Oliveira Trindade A; Escola Superior de Ciências da Saúde, Centro Multiusuário para Análise de Fenômenos Biomédicos da Universidade do Estado do Amazonas, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Carlos Salazar-Alvarez L; Departamento de Morfologia, Universidade Federal do Amazonas, Manaus, Brazil.
  • Costa Ferreira Neves J; Instituto Leônidas & Maria Deane, Fiocruz Amazônia, Manaus, Brazil.
  • Dos Santos Bastos I; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil.
  • Guimarães Costa A; Departamento de Saúde Coletiva, Universidade Federal do Amazonas, Manaus, Brazil.
  • Vinicius Guimarães Lacerda M; Faculdade de Farmácia, Universidade Nilton Lins, Manaus, Brazil.
  • Marcelo Monteiro W; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Trindade Maranhão Costa F; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil.
  • Costa Pinto Lopes S; Departamento de Genética, Evolução, Microbiologia e Imunologia, Universidade Estadual de Campinas, Campinas, Brazil.
Front Cell Infect Microbiol ; 13: 1108366, 2023.
Article em En | MEDLINE | ID: mdl-37143740
ABSTRACT
Methylene blue (MB) is an alternative for combating drug-resistant malaria parasites. Its transmission-blocking potential has been demonstrated in vivo in murine models, in vitro, and in clinical trials. MB shows high efficacy against Plasmodium vivax asexual stages; however, its efficacy in sexual stages is unknown. In this study, we evaluated the potential of MB against asexual and sexual forms of P. vivax isolated from the blood of patients residing in the Brazilian Amazon. An ex vivo schizont maturation assay, zygote to ookinete transformation assay, direct membrane feed assay (DMFA), and standard membrane feed assay (SMFA) using P. vivax gametocytes with MB exposure were performed. A cytotoxicity assay was also performed on freshly collected peripheral blood mononuclear cells (PBMCs) and the hepatocyte carcinoma cell line HepG2. MB inhibited the P. vivax schizont maturation and demonstrated an IC50 lower than that of chloroquine (control drug). In the sexual forms, the MB demonstrated a high level of inhibition in the transformation of the zygotes into ookinetes. In the DMFA, MB did not considerably affect the infection rate and showed low inhibition, but it demonstrated a slight decrease in the infection intensity in all tested concentrations. In contrast, in the SMFA, MB was able to completely block the transmission at the highest concentration (20 µM). MB demonstrated low cytotoxicity to fresh PBMCs but demonstrated higher cytotoxicity to the hepatocyte carcinoma cell line HepG2. These results show that MB may be a potential drug for vivax malaria treatment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma / Malária Vivax Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma / Malária Vivax Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article