Protocol for tumor dissociation and fluorescence-activated cell sorting of human head and neck cancers.
STAR Protoc
; 4(2): 102294, 2023 May 06.
Article
em En
| MEDLINE
| ID: mdl-37149858
ABSTRACT
Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh human head and neck tumor specimens, followed by isolation of viable single cells using fluorescence-activated cell sorting. Our protocol facilitates the effective downstream use of techniques, including single-cell RNA sequencing and generation of three-dimensional patient-derived organoids. For complete details on the use and execution of this protocol, please refer to Puram et al. (2017)1 and Parikh et al. (2022).2.
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MEDLINE
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En
Ano de publicação:
2023
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Article