Complex regulation of Eomes levels mediated through distinct functional features of the Meteor long non-coding RNA locus.
Cell Rep
; 42(6): 112569, 2023 06 27.
Article
em En
| MEDLINE
| ID: mdl-37256750
ABSTRACT
Long non-coding RNAs (lncRNAs) are implicated in a plethora of cellular processes, but an in-depth understanding of their functional features or their mechanisms of action is currently lacking. Here we study Meteor, a lncRNA transcribed near the gene encoding EOMES, a pleiotropic transcription factor implicated in various processes throughout development and in adult tissues. Using a wide array of perturbation techniques, we show that transcription elongation through the Meteor locus is required for Eomes activation in mouse embryonic stem cells, with Meteor repression linked to a change in the subpopulation primed to differentiate to the mesoderm lineage. We further demonstrate that a distinct functional feature of the locus-namely, the underlying DNA element-is required for suppressing Eomes expression following neuronal differentiation. Our results demonstrate the complex regulation that can be conferred by a single locus and emphasize the importance of careful selection of perturbation techniques when studying lncRNA loci.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas com Domínio T
/
RNA Longo não Codificante
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article