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A Scanning Electron Microscope Evaluation of the Adhesion of Fibrin Clot to the Periodontally Compromised Teeth after Exposed to Different Root-conditioning Agents: An In Vitro Study.
Verma, Abhishek; Raj, Kavita; Satyanarayan, Ashok; Archana, Devanoorkar; Anand, Kumar; Nath, Barsha.
Afiliação
  • Verma A; Department of Periodontology and Implantology, Buddha Institute of Dental Sciences & Hospital, Patna, Bihar, India, Phone: +91 9546150797, e-mail: drverma_mds@rediffmail.com.
  • Raj K; Department of Dentistry, Gandhi Medical College, Bhopal, Madhya Pradesh, India.
  • Satyanarayan A; Department of Dentistry, Gulbarga Institute of Medical Sciences, Kalaburagi, Karnataka, India.
  • Archana D; Department of Dentistry, Gulbarga Institute of Medical Sciences, Kalaburagi, Karnataka, India.
  • Anand K; Department of Oral Medicine and Radiology, Buddha Institute of Dental Sciences and Hospital, Patna, Bihar, India.
  • Nath B; Department of Periodontology and Implantology, Buddha Institute of Dental Sciences & Hospital, Patna, Bihar, India.
J Contemp Dent Pract ; 23(9): 895-899, 2022 Sep 01.
Article em En | MEDLINE | ID: mdl-37282996
ABSTRACT

AIM:

The aim of this research was to assess the binding of fibrin clot to the teeth affected by periodontal disease following exposure to different root conditioning agents. MATERIALS AND

METHODS:

A total of 60 human teeth with a solitary root that were subjected to extraction following severe periodontal disease were used as study samples in this research. Two analogous grooves were prepared on the proximal radicular surface of every sample employing a diamond-tapered fissure bur using an aerator handpiece beneath abundant irrigation. Every sample was assigned to one of the following groups • Group I Tetracycline hydrochloride solution • Group II Ethylenediaminetetraacetic acid (EDTA) gel • Group III Biopure MTAD™ Subsequent to conditioning, the samples were rinsed for 3 minutes with phosphate-buffered saline (PBS) and permitted to air-dry for 20 minutes. A drop of fresh human whole blood procured from a hale and hearty volunteer was coated onto the dentin blocks in all three groups. A scanning electron microscope under 5000× magnification at 15 kV was used to examine the samples. Kruskal-Wallis test and Mann-Whitney U test were performed to procure the inter- and intragroup assessments

Results:

The greatest fibrin clot union was noted in the EDTA gel group at 2.86 ± 0.14 in pursuit by Biopure MTAD™ group at 2.39 ± 0.08 as well as tetracycline hydrochloride solution group at 1.82 ± 0.10. A statistically significant difference was noted between the investigational groups (p < 0.001).

CONCLUSION:

This research arrived at a conclusion that the dentinal surfaces subjected to conditioning with EDTA gel group as well as coated with human whole blood resulted in appreciably superior fibrin clot bonding to dentin vs Biopure MTAD™ as well as the tetracycline hydrochloride solution group. CLINICAL

SIGNIFICANCE:

Connective tissue attachment subsequent to surgical procedures causing the adhesion of a fibrin clot to the radicular surface as a result of initial wound healing processes is directly related to periodontal regeneration. It depends on biocompatibility for the fibrin clot and the periodontal pathosis-affected radicular surface to stick together, which can be procured with the aid of a variety of root conditioning measures in course of periodontal treatment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Periodontite / Trombose / Camada de Esfregaço Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Periodontite / Trombose / Camada de Esfregaço Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article