Optimization of multi-enzyme cascade process for the biosynthesis of benzylamine.
Biosci Biotechnol Biochem
; 87(11): 1373-1380, 2023 Oct 25.
Article
em En
| MEDLINE
| ID: mdl-37567780
ABSTRACT
Benzylamine is a valuable intermediate in the synthesis of organic compounds such as curing agents and antifungal drugs. To improve the efficiency of benzylamine biosynthesis, we identified the enzymes involved in the multi-enzyme cascade, regulated the expression strength by using RBS engineering in Escherichia coli, and established a regeneration-recycling system for alanine. This is a cosubstrate, coupled to cascade reactions, which resulted in E. coli RARE-TP and can synthesize benzylamine using phenylalanine as a precursor. By optimizing the supply of cosubstrates alanine and ammonia, the yield of benzylamine produced by whole-cell catalysis was increased by 1.5-fold and 2.7-fold, respectively, and the final concentration reached 6.21 mM. In conclusion, we achieved conversion from l-phenylalanine to benzylamine and increased the yield through enzyme screening, expression regulation, and whole-cell catalytic system optimization. This demonstrated a green and sustainable benzylamine synthesis method, which provides a reference and additional information for benzylamine biosynthesis research.
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Base de dados:
MEDLINE
Assunto principal:
Benzilaminas
/
Escherichia coli
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article