Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.
Methods Mol Biol
; 2710: 121-129, 2023.
Article
em En
| MEDLINE
| ID: mdl-37688729
ABSTRACT
The olfactory mucosa, lining a portion of the nasal cavity, houses the primary olfactory sensory neurons responsible for odor transduction, along with supporting cell populations. Tremendous advances have come from studying the peripheral olfactory system in animal models, especially the mouse. However, acquired human olfactory disorders lack effective therapies, and many of these conditions involve pathology in the olfactory mucosa. Thus, the ability to obtain human olfactory biopsy samples from subjects with olfactory dysfunction, or controls, may be of value. Here, we describe established techniques for collecting olfactory tissue from human subjects and preparing samples for downstream assays such as immunohistochemistry, flow cytometry, single-cell RNA-sequencing, or chromatin studies.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Olfato
/
Bioensaio
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article