Conservation of Chi cutting activity in terrestrial and marine enteric bacteria.
J Mol Biol
; 189(4): 585-95, 1986 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-3783685
ABSTRACT
Homologous recombination in Escherichia coli occurs at increased frequency near Chi sites, 5'G-C-T-G-G-T-G-G3'. Cutting of DNA close to the Chi sequence by the E. coli RecBC enzyme is essential to Chi's stimulation of recombination. We have detected Chi-dependent cutting activity in extracts of several genera of terrestrial enteric bacteria (family Enterobacteriaceae) and of two genera of marine enteric bacteria (family Vibrionaceae). More distantly related bacteria had no detectable Chi-dependent cutting activity. These results support the view that recognition of this specific nucleotide sequence as a signal activating recombination has been maintained during the evolution of certain groups of bacteria. We discuss the possibility that other sequences play a similar role in other groups of bacteria.
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Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Vibrionaceae
/
Enterobacteriaceae
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article