Epigenetic regulation limits competence of pluripotent stem cell-derived oocytes.
EMBO J
; 42(23): e113955, 2023 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-37850882
ABSTRACT
Recent studies have reported the differentiation of pluripotent cells into oocytes in vitro. However, the developmental competence of in vitro-generated oocytes remains low. Here, we perform a comprehensive comparison of mouse germ cell development in vitro over all culture steps versus in vivo with the goal to understand mechanisms underlying poor oocyte quality. We show that the in vitro differentiation of primordial germ cells to growing oocytes and subsequent follicle growth is critical for competence for preimplantation development. Systematic transcriptome analysis of single oocytes that were subjected to different culture steps identifies genes that are normally upregulated during oocyte growth to be susceptible for misregulation during in vitro oogenesis. Many misregulated genes are Polycomb targets. Deregulation of Polycomb repression is therefore a key cause and the earliest defect known in in vitro oocyte differentiation. Conversely, structurally normal in vitro-derived oocytes fail at zygotic genome activation and show abnormal acquisition of 5-hydroxymethylcytosine on maternal chromosomes. Our data identify epigenetic regulation at an early stage of oogenesis limiting developmental competence and suggest opportunities for future improvements.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Epigênese Genética
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article