Usefulness of current sgRNA design guidelines and in vitro cleavage assays for plant CRISPR/Cas genome editing: a case targeting the polyphenol oxidase gene family in eggplant (Solanum melongena L.).

Sagarbarria, Mark Gabriel S; Caraan, John Albert M; Layos, Angelo John G

Sagarbarria, Mark Gabriel S; Caraan, John Albert M; Layos, Angelo John G.

Afiliação

Sagarbarria MGS; Genetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines. mssagarbarria@up.edu.ph.
Caraan JAM; Genetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines.
Layos AJG; Genetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines.

Transgenic Res ; 32(6): 561-573, 2023 12.

Article em En | MEDLINE | ID: mdl-37874448

Assuntos

Edição de Genes; Solanum melongena; Animais; RNA Guia de Sistemas CRISPR-Cas; Sistemas CRISPR-Cas/genética; Solanum melongena/genética; Genoma de Planta/genética

Palavras-chave

CRISPR/Cas9; Eggplant; In vitro assay; In vivo genome editing; Single guide RNA

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Solanum melongena / Edição de Genes Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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