Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition.
Biochem Biophys Res Commun
; 694: 149395, 2024 01 29.
Article
em En
| MEDLINE
| ID: mdl-38141557
ABSTRACT
SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVMP9A were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving Ni-NTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVMP9A) and SpoIVA in Bicelle conditions.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Trifosfato de Adenosina
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article