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Comparative analysis between mercury levels in fish tissues evaluated using direct mercury analyzer and inductively plasma-coupled mass spectrometer.
Nunes Neto, Osvaldo Gato; Dias, Salatiel Ribeiro; Albuquerque, Fábio Edir Amaral; Miranda, Marta; Lopez-Alonso, Marta; Oliveira, Ricardo Bezerra; Pinto, Diana; Minervino, Antonio Humberto Hamad.
Afiliação
  • Nunes Neto OG; Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Brazil; Ph.D. Program in Society, Nature and Development, PPGSND, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Br
  • Dias SR; Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Brazil; Ph.D. Program in Society, Nature and Development, PPGSND, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Br
  • Albuquerque FEA; Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Brazil; Department of Animal Pathology, Veterinary Faculty, University of Santiago de Compostela, 27002, Lugo, Spain.
  • Miranda M; Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Veterinary Faculty, University of Santiago de Compostela, 27002, Lugo, Spain.
  • Lopez-Alonso M; Department of Animal Pathology, Veterinary Faculty, University of Santiago de Compostela, 27002, Lugo, Spain.
  • Oliveira RB; Bioprospecting and Experimental Biology Laboratory. Institute of Education Sciences. Federal University of Western Pará, UFOPA. Av. Marechal Rondon s/n, 68040-070, Brazil. Electronic address: ricardo.oliveira@ufopa.edu.br.
  • Pinto D; Universidad de la Costa, CUC, Calle 58 # 55-66, Barranquilla, 080002, Colombia.
  • Minervino AHH; Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Brazil; Ph.D. Program in Society, Nature and Development, PPGSND, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n, Salé, CEP 68040-255, Santarém, PA, Br
Chemosphere ; 351: 141146, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38211792
ABSTRACT
Recent ecotoxicological studies have indicated mercury (Hg) contamination in aquatic ecosystems in the Amazon Basin. Although Hg contamination can be associated with small-scale gold mining, the soils of the Amazon region have naturally high Hg concentrations, and can be transported to aquatic ecosystems via deforestation and mining activities. Biomagnification of Hg can pose risks to the local human population; therefore, its concentration in fish tissues must be monitored consistently. Fast and sensitive Hg determination is required for continuously monitoring ecosystems impacted by mineral exploration. The direct mercury analyzer (DMA-80) is widely used for determining total Hg levels in tissue samples; it is fast and cost-effective, without requiring sample preparation. Here, we determined the sensitivity and specificity of Hg detection accomplished using DMA-80, and whether these results are reliable compared to those obtained using Inductively Coupled Plasma Mass Spectrometer (ICP-MS), which is the gold standard. We obtained 106 paired dried samples of muscle tissue from fish species occupying different trophic levels in the Lower Amazon region, and analyzed them using both equipment (DMA-80 and ICP-MS). The results obtained using DMA-80 had an overall Hg mean of 1.90 ± 0.18 mg/kg which was higher (p < 0.05) than the mean of those obtained using ICP-MS (1.55 ± 0.13 mg/kg). Linear regression analysis comparing the Hg levels obtained using both devices was within the 95% prediction interval, and a high coefficient of correlation showed agreement between the devices (r = 0.979; 0.069 to 0.986, 95% CI). Bland-Altman analysis showed that DMA-80 had a positive bias of 6.5% in relation to ICP-MS, which is more evident in samples with high Hg concentrations. DMA-80 was efficient in determining whether the Hg levels exceeded the maximum allowed levels required by the European Union, USA, and Brazil, showing a specificity and sensitivity of above 95%.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mercúrio Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mercúrio Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article