Leveraging a Y. lipolytica naringenin chassis for biosynthesis of apigenin and associated glucoside.
Metab Eng
; 83: 1-11, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38447910
ABSTRACT
Flavonoids are a diverse set of natural products with promising bioactivities including anti-inflammatory, anti-cancer, and neuroprotective properties. Previously, the oleaginous host Yarrowia lipolytica has been engineered to produce high titers of the base flavonoid naringenin. Here, we leverage this host along with a set of E. coli bioconversion strains to produce the flavone apigenin and its glycosylated derivative isovitexin, two potential nutraceutical and pharmaceutical candidates. Through downstream strain selection, co-culture optimization, media composition, and mutant isolation, we were able to produce168 mg/L of apigenin, representing a 46% conversion rate of 2-(R/S)-naringenin to apigenin. This apigenin platform was modularly extended to produce isovitexin by addition of a second bioconversion strain. Together, these results demonstrate the promise of microbial production and modular bioconversion to access diversified flavonoids.
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Base de dados:
MEDLINE
Assunto principal:
Yarrowia
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Flavanonas
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Apigenina
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Escherichia coli
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Engenharia Metabólica
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article