Catalytic hairpin self-assembly amplification fluorescence detection of chloramphenicol based on cross-shaped DNA and UiO-66.
Spectrochim Acta A Mol Biomol Spectrosc
; 313: 124110, 2024 May 15.
Article
em En
| MEDLINE
| ID: mdl-38452462
ABSTRACT
A catalytic hairpin self-assembly (CHA) amplification method was developed for CAP detection based on cross-shaped DNA and UiO-66. MOF was used to quench the fluorescent signal of FAM labeled DNA. Cross-shaped DNA with four fluorophore group (FAM) was utilized to enhance the fluorescent intensity. CAP could open hairpin structure of H-apt and induce CHA reaction. The product of CHA hybridized with cross-shaped DNA, resulting its leaving from the surface of UiO-66 and recovery of fluorescent signal. The limit of detection (LOD) was low to 0.87 pM. This method had been successfully applied for the detection of CAP in actual samples. Importantly, the high sensitivity was attributed to the great amplification efficiency of CHA, strong fluorescent intensity of cross-shaped DNA structure and great fluorescent quenched efficiency of UiO-66.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Ftálicos
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Técnicas Biossensoriais
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DNA Catalítico
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Estruturas Metalorgânicas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article