Structural basis of human U5 snRNP late biogenesis and recycling.
Nat Struct Mol Biol
; 31(5): 747-751, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38467876
ABSTRACT
Pre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP is the ~1 MDa 'heart' of the spliceosome and is recycled through an unknown mechanism involving major architectural rearrangements and the dedicated chaperones CD2BP2 and TSSC4. Late steps in U5 snRNP biogenesis similarly involve these chaperones. Here we report cryo-electron microscopy structures of four human U5 snRNP-CD2BP2-TSSC4 complexes, revealing how a series of molecular events primes the U5 snRNP to generate the ~2 MDa U4/U6.U5 tri-snRNP, the largest building block of the spliceosome.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Modelos Moleculares
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Spliceossomos
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Ribonucleoproteína Nuclear Pequena U5
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Microscopia Crioeletrônica
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article