Metallomic mapping of gut and brain in heavy metal exposed earthworms: A novel paradigm in ecotoxicology.
Biochem Biophys Res Commun
; 709: 149827, 2024 05 21.
Article
em En
| MEDLINE
| ID: mdl-38554600
ABSTRACT
This study explored the uptake of lead in the epigeic earthworm Dendrobaena veneta exposed to 0, 1000, and 2500 µg Pb/g soil. The soil metal content was extracted using strong acid digestion and water leaching, and analysed by means of Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to estimate absolute and bioavailable concentrations of metals in the soil. The guts and heads of lead-exposed earthworms were processed into formalin-fixed and paraffin embedded sections for high-resolution multi-element metallomic imaging via Laser Ablation ICP-MS (LA-ICP-MS). Metallomic maps of phosphorus, zinc, and lead were produced at 15-µm resolution in the head and gut of D. veneta. Additional 4-µm resolution metallomic maps of the earthworm brains were taken, revealing the detailed localisation of metals in the brain. The Pb bioaccumulated in the chloragogenous tissues of the earthworm in a dose-dependent manner, making it possible to track the extent of soil contamination. The bioaccumulation of P and Zn in earthworm tissues was independent of Pb exposure concentration. This approach demonstrates the utility of LA-ICP-MS as a powerful approach for ecotoxicology and environmental risk assessments.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligoquetos
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Poluentes do Solo
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Metais Pesados
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article