Wireless rotating bipolar electrochemiluminescence for enzymatic detection.
Analyst
; 149(9): 2756-2761, 2024 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-38563766
ABSTRACT
New dynamic, wireless and cost-effective analytical devices are developing rapidly in biochemical analysis. Here, we report on a remotely-controlled rotating electrochemiluminescence (ECL) sensing system for enzymatic detection of a model analyte, glucose, on both polarized sides of an iron wire acting as a bipolar electrode. The iron wire is controlled by double contactless mode, involving remote electric field polarization, and magnetic field-induced rotational motion. The former triggers the interfacial polarization of both extremities of the wire by bipolar electrochemistry, which generates ECL emission of the luminol derivative (L-012) with the enzymatically produced hydrogen peroxide in presence of glucose, at both anodic and cathodic poles, simultaneously. The latter generates a convective flow, leading to an increase in mass transfer and amplifying the corresponding ECL signals. Quantitative glucose detection in human serum samples is achieved. The ECL signals were found to be a linear function of the glucose concentration within the range of 10-1000 µM and with a limit of detection of 10 µM. The dynamic bipolar ECL system simultaneously generates light emissions at both anodic and cathodic poles for glucose detection, which can be further applied to biosensing and imaging in autonomous devices.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Eletroquímicas
/
Medições Luminescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article