Humanin's impact on pain markers and neuronal viability in diabetic neuropathy model.
Arch Physiol Biochem
; : 1-11, 2024 Apr 10.
Article
em En
| MEDLINE
| ID: mdl-38599217
ABSTRACT
OBJECTIVE:
This study investigates the impact of chronic humanin (HN) treatment on pain-related markers (NMDA, substance P, TRPV1, and IL-1ß) in diabetic mice's dorsal root ganglia (DRG). Additionally, we assess the effects of HN on cellular viability in DRG neurons.METHODS:
In vivo experiments involved 15 days of HN administration (4 mg/kg) to diabetic mice (n = 10). Protein levels of NMDA, IL-1ß, TRPV1, and substance P were measured in diabetic DRG. In vitro experiments explored HN's impact on apoptosis and cellular viability, focusing on the JAK2/STAT3 pathway.RESULTS:
Humanin significantly reduced the elevated expression of NMDA, IL-1ß, TRPV1, and substance P induced by diabetes (p < .05). Furthermore, HN treatment increased cellular viability in DRG neurons through JAK2/STAT3 pathway activation (p < .05).CONCLUSION:
These findings highlight the significance of understanding mitochondrial function and pain markers, as well as apoptosis in diabetes. The study provides insights for managing the condition and its complications.
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MEDLINE
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article