Reconstructing Signaling Networks Using Biosensor Barcoding.
Methods Mol Biol
; 2800: 189-202, 2024.
Article
em En
| MEDLINE
| ID: mdl-38709485
ABSTRACT
Understanding how signaling networks are regulated offers valuable insights into how cells and organisms react to internal and external stimuli and is crucial for developing novel strategies to treat diseases. To achieve this, it is necessary to delineate the intricate interactions between the nodes in the network, which can be accomplished by measuring the activities of individual nodes under perturbation conditions. To facilitate this, we have recently developed a biosensor barcoding technique that enables massively multiplexed tracking of numerous signaling activities in live cells using genetically encoded fluorescent biosensors. In this chapter, we detail how we employed this method to reconstruct the EGFR signaling network by systematically monitoring the activities of individual nodes under perturbations.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Transdução de Sinais
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article