Preparation and application of fluorescent monoclonal antibodies recognizing goat CD4+CD25+ regulatory T cells.
Appl Microbiol Biotechnol
; 108(1): 327, 2024 May 08.
Article
em En
| MEDLINE
| ID: mdl-38717623
ABSTRACT
Regulatory T cells (Tregs) are a subset of T cells participating in a variety of diseases including mycoplasmal pneumonia, contagious ecthyma, and so on. The role of Tregs in goat contagious ecthyma is not completely understood due to the lack of species-specific antibodies. Here, we developed a combination of CD4 and CD25 fluorescence monoclonal antibodies (mAb) to recognize goat Tregs and assessed its utility in flow cytometry, immunofluorescence staining. Using immunofluorescence staining, we found that the frequency of Treg cells was positively correlated with the viral load during orf virus infection. These antibodies could serve as important tools to monitor Tregs during orf virus infection in goats. KEY POINTS ⢠A combination of fluorescent mAbs (C11 and D12) was prepared for the detection of goat Tregs. ⢠C11 and D12 are effective in flow cytometry, immunofluorescence staining, and C11 has excellent species specificity. ⢠The frequency of Treg cells was positively correlated with the viral load during orf virus infection.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cabras
/
Linfócitos T Reguladores
/
Carga Viral
/
Citometria de Fluxo
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article