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Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat.
Zhang, Meng-Qian; Yang, Zhi; Dong, Yu-Xin; Zhu, Ya-Li; Chen, Xin-Yi; Dai, Chuan-Chao; Zhichun, Zhan; Mei, Yan-Zhen.
Afiliação
  • Zhang MQ; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China.
  • Yang Z; Wuhan Sunhy Biology Co., Ltd.,Wuhan, 430000, Hubei, China.
  • Dong YX; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China.
  • Zhu YL; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China.
  • Chen XY; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China.
  • Dai CC; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China.
  • Zhichun Z; Wuhan Sunhy Biology Co., Ltd.,Wuhan, 430000, Hubei, China.
  • Mei YZ; Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023 Jiangsu, China. Electronic address: meiyanzhen@njnu.edu.cn.
Fungal Genet Biol ; 173: 103899, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38802054
ABSTRACT
Fusarium head blight is a devastating disease that causes severe yield loses and mycotoxin contamination in wheat grain. Additionally, balancing the trade-off between wheat production and disease resistance has proved challenging. This study aimed to expand the genetic tools of the endophyte Phomopsis liquidambaris against Fusarium graminearum. Specifically, we engineered a UDP-glucosyltransferase-expressing P. liquidambaris strain (PL-UGT) using ADE1 as a selection marker and obtained a deletion mutant using an inducible promoter that drives Cas9 expression. Our PL-UGT strain converted deoxynivalenol (DON) into DON-3-G in vitro at a rate of 71.4 % after 36 h. DON inactivation can be used to confer tolerance in planta. Wheat seedlings inoculated with endophytic strain PL-UGT showed improved growth compared with those inoculated with wildtype P. liquidambaris. Strain PL-UGT inhibited the growth of Fusarium graminearum and reduced infection rate to 15.7 %. Consistent with this finding, DON levels in wheat grains decreased from 14.25 to 0.56 µg/g when the flowers were pre-inoculated with PL-UGT and then infected with F. graminearum. The expression of UGT in P. liquidambaris was nontoxic and did not inhibit plant growth. Endophytes do not enter the seeds nor induce plant disease, thereby representing a novel approach to fungal disease control.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ascomicetos / Tricotecenos / Triticum / Endófitos / Fusarium / Glucosiltransferases Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ascomicetos / Tricotecenos / Triticum / Endófitos / Fusarium / Glucosiltransferases Idioma: En Ano de publicação: 2024 Tipo de documento: Article