The relationship between nanoscale genome organization and gene expression in mouse embryonic stem cells during pluripotency transition.
Nucleic Acids Res
; 52(14): 8146-8164, 2024 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-38850157
ABSTRACT
During early development, gene expression is tightly regulated. However, how genome organization controls gene expression during the transition from naïve embryonic stem cells to epiblast stem cells is still poorly understood. Using single-molecule microscopy approaches to reach nanoscale resolution, we show that genome remodeling affects gene transcription during pluripotency transition. Specifically, after exit from the naïve pluripotency state, chromatin becomes less compacted, and the OCT4 transcription factor has lower mobility and is more bound to its cognate sites. In epiblast cells, the active transcription hallmark, H3K9ac, decreases within the Oct4 locus, correlating with reduced accessibility of OCT4 and, in turn, with reduced expression of Oct4 nascent RNAs. Despite the high variability in the distances between active pluripotency genes, distances between Nodal and Oct4 decrease during epiblast specification. In particular, highly expressed Oct4 alleles are closer to nuclear speckles during all stages of the pluripotency transition, while only a distinct group of highly expressed Nodal alleles are in close proximity to Oct4 when associated with a nuclear speckle in epiblast cells. Overall, our results provide new insights into the role of the spatiotemporal genome remodeling during mouse pluripotency transition and its correlation with the expression of key pluripotency genes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma
/
Fator 3 de Transcrição de Octâmero
/
Células-Tronco Embrionárias Murinas
/
Camadas Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article