Detection of MicroRNA-155 based on lambda exonuclease selective digestion and CRISPR/cas12a-assisted amplification.

Zhang, Haotian; Xu, Jun; Liu, Shiwen; Li, Hongbo; Xu, Lianlian; Wang, Suqin

Zhang, Haotian; Xu, Jun; Liu, Shiwen; Li, Hongbo; Xu, Lianlian; Wang, Suqin.

Afiliação

Zhang H; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.
Xu J; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.
Liu S; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China; Jiangxi Provincial Center for Disease Control and Prevention, Nanchang, 330029, PR China. Electronic addr
Li H; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China. Electronic address: lihongbo112@126.com.
Xu L; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.
Wang S; Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.

Anal Biochem ; 693: 115592, 2024 Oct.

Article em En | MEDLINE | ID: mdl-38871161

Assuntos

Técnicas Biossensoriais; Sistemas CRISPR-Cas; MicroRNAs; MicroRNAs/genética; MicroRNAs/sangue; MicroRNAs/análise; Humanos; Técnicas Biossensoriais/métodos; Sistemas CRISPR-Cas/genética; Proteínas Associadas a CRISPR/metabolismo; Proteínas Associadas a CRISPR/genética; Técnicas de Amplificação de Ácido Nucleico/métodos; Limite de Detecção; Proteínas de Bactérias/genética; Proteínas de Bactérias/metabolismo; Bacteriófago lambda/genética; Endodesoxirribonucleases

Palavras-chave

CRISPR/Cas12a; Fluorescence detection; Hairpin probe; MiRNA-155; Trans-cutting

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs / Sistemas CRISPR-Cas Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

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