Single cell transcriptome analysis identified a unique neutrophil type associated with Alzheimer's disease.

Zhang, Xiaolin; He, Guiqin; Hu, Yixuan; Liu, Boren; Xu, Yuliang; Li, Xia; Lv, Xinyou; Li, Jin

Zhang, Xiaolin; He, Guiqin; Hu, Yixuan; Liu, Boren; Xu, Yuliang; Li, Xia; Lv, Xinyou; Li, Jin.

Afiliação

Zhang X; Shanghai Key Laboratory of Psychotic Disorders, Brain Health Institute, National Center for Mental Disorders, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai, 200030, China.
He G; Shanghai Key Laboratory of Psychotic Disorders, Brain Health Institute, National Center for Mental Disorders, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai, 200030, China.
Hu Y; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201210, China.
Liu B; University of Chinese Academy of Sciences, Beijing, China.
Xu Y; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Li X; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Lv X; Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.
Li J; Department of Psychology, School of Humanities and Social Sciences, University of Science and Technology of China, Hefei, 230026, Anhui, China. xinyoulv@ustc.edu.cn.

Immun Ageing ; 21(1): 42, 2024 Jun 25.

Article em En | MEDLINE | ID: mdl-38918830

ABSTRACT

ABSTRACT

BACKGROUND:

Neutrophils play an essential role in Alzheimer's disease (AD) pathology. However, the extent of their heterogeneity remains poorly explored, particularly in the context of developing novel therapies targeting these cells.

RESULTS:

We investigate the population structure of neutrophils purified from peripheral blood samples of AD mice. Utilizing single cell RNA sequencing, we comprehensively map neutrophil populations into six distinct clusters and find that the Neu-5 subset is specially enriched in AD mice. This subset exhibits fewer specific granules and a lower mature score. Gene ontology (GO) analysis reveals that genes involved in cytokine-mediated signaling are downregulated in the Neu-5 cluster. Furthermore, we identify the Ccrl2 gene is specifically upregulated in this subgroup, which is confirmed by flow cytometry in AD mice. Finally, immunohistochemical staining indicates that CCRL2 protein is increased in the brains of AD mice.