A novel dual-recognition fluorescent biosensor for sialyl-Lewisx sensitive detection.
Mikrochim Acta
; 191(8): 479, 2024 07 23.
Article
em En
| MEDLINE
| ID: mdl-39042166
ABSTRACT
Sialyl-Lewisx (SLex) is a tetrasugar, which plays an important role in initial inflammation and cancer cell metastasis, and can be used as a marker for cancer diagnosis and prognosis or a therapeutic target. Detecting SLex from complex biological media remains a significant challenge. Herein, a single-stranded DNA aptamer of SLex was screened based on the double-stranded DNA library-modified magnetic bead (MB)-SELEX technology. After 14 rounds of screening, 12,639 sequences were obtained and divided into nine families. Three representative sequences were selected based on the number of sequence repeats and Gibbs binding free energy, and the aptamer SLex-Apt2 with 80 nt length (Kd = 23.01 nM) had the best affinity and relatively high specificity for targeting SLex. Then, a novel dual-recognition fluorescent biosensor for SLex-sensitive detection based on aptamer SLex-Apt2 bio-dots and 3-aminobenzoboric acid-modified MB was developed. This method can detect SLex as low as 32 µM and has a good linear response in the range 100 µM to 2 mM. It has the advantages of low preparation cost, good targeting, and avoiding the occurrence of false-positive and false-negative detection results, which makes the biosensor more valuable in biological detection and clinical diagnosis.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Aptâmeros de Nucleotídeos
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Técnica de Seleção de Aptâmeros
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Antígeno Sialil Lewis X
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article