Fungicide Metabolite MS2 Spectral Libraries for Comprehensive Human Biomonitoring.
J Agric Food Chem
; 72(32): 18247-18256, 2024 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-39101478
ABSTRACT
Fungicides undergo rapid metabolism and are excreted in the urine. There are few methods for screening these ubiquitous compounds, which have a high potential for human exposure. High-resolution mass spectrometry (HRMS) is a suitable technique to assess fungicide exposures; however, there is a lack of spectral libraries for fungicide annotation and in particular for downstream metabolites. We created spectral libraries for 32 fungicides for suspect screening. Fungicide standards were administered to mice, and 24-h urine was analyzed using hydrophilic interaction and reversed-phase chromatography coupled to hybrid quadrupole-orbitrap mass spectrometry. Suspect metabolite MS2 spectra for library creation were selected based on the ratio of exposed-to-control mouse urine. MS2 libraries were applied to urine collected from female university students (n = 73). Several tetraconazole and tebuconazole metabolites were detected in 3% (2/73) of the samples. The creation of comprehensive suspect screening MS2 libraries is a useful tool to detect fungicide exposure for human biomonitoring.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fungicidas Industriais
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Monitoramento Biológico
Limite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article