Primary human mesenchymal stem cell culture under xeno-free conditions using surface-modified cellulose nanofiber scaffolds.
Carbohydr Polym
; 343: 122479, 2024 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-39174138
ABSTRACT
Stem cell culture often requires various animal-derived components such as serum and collagen. This limits its practical use. Therefore, xeno-free (xenogeneic component-free) culture systems are receiving increased attention. Herein, we propose xeno-free, plant-derived cellulose nanofibers (CNFs) with different surface chemistry 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized CNFs (TOCNFs) with carboxy groups and surface-sulfated CNFs (S-CNFs) for the proliferation of human mesenchymal stem cells (hMSCs) under various serum conditions. We cultured bone marrow-derived hMSCs on CNF scaffolds with various fiber lengths and functional group contents. Original CNFs were bioinert materials that did not contribute to cell adhesion. In contrast, the surface-modified CNFs facilitated the proliferation of immortalized hMSCs under normal and low-serum conditions. The TOCNFs (COONa 1.47 mmol g-1; length 0.53 µm), the S-CNFs (OSO3Na 0.64 mmol g-1; 0.61 µm), and a combination of the two (11 by weight) enabled immortalized hMSCs to maintain their multipotency, even under serum-free conditions. Primary cultured hMSCs proliferated well on the TOCNF/S-CNF scaffolds in a completely serum-free medium, comparable to animal-derived type I collagen, although few hMSCs adhered to the standard polystyrene substrate. Our strategy of using surface-modified CNFs will inform the development of xeno-free culture systems to avoid the use of animal-derived materials for both cell culture media and scaffolds.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Celulose
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Proliferação de Células
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Alicerces Teciduais
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Nanofibras
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Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article