Histones in the first cleavage cycle of fertilised sea urchin eggs.
Cell Differ
; 7(5): 271-81, 1978 Oct.
Article
em En
| MEDLINE
| ID: mdl-568032
ABSTRACT
Nuclei were isolated from Echinus eggs through the first cleavage cycle by modification of existing techniques. When these nuclei were extracted with 2 M NaCl and the supernatant diluted to 0.15 M, large amounts of non-histone proteins remained in solution. The precipitated nucleoprotein contained expected amounts of DNA and a protein analogous to mammalian histone H1. Extrachromosomal histone H1 was eliminated by the modified isolation procedure. Amounts of nuclear proteins soluble in 0.15 M NaCl reached a peak in G2. Histones and non-histone proteins were phosphorylated postfertilization, in early prophase and in telophase.
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Base de dados:
MEDLINE
Assunto principal:
Zigoto
/
Histonas
Limite:
Animals
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article