Enzyme-linked immunosorbent assay for detection of specific antibodies to Ureaplasma urealyticum serotypes.
J Clin Microbiol
; 19(3): 421-6, 1984 Mar.
Article
em En
| MEDLINE
| ID: mdl-6201503
ABSTRACT
Optimal conditions of a micro-enzyme-linked immunosorbent assay system for the detection of immunoglobulin G antibodies to Ureaplasma urealyticum were established with rabbit antisera. Initially, the antisera, raised against eight U. urealyticum serotypes grown on medium containing horse serum, displayed nonspecific reactions with our enzyme-linked immunosorbent assay antigens. Substitution of fetal bovine serum in the medium eliminated this nonspecificity. The assay was then serotype-specific for the original eight U. urealyticum serotypes. The prominent homologous reaction was easily differentiated from the heterologous reactions. A one-way cross-reaction was observed with serotype 2 antiserum and serotype 5 antigen. The results were reproducible and could be obtained in 4 h with only 10 microliters of serum for eight serotypes. Optimal antigen concentrations for the U. urealyticum serotypes ranged from 0.40 to 1.60 micrograms/ml. Our results indicated that enzyme-linked immunosorbent assay has the potential for the detection of antibodies to specific serotypes of U. urealyticum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ureaplasma
/
Ensaio de Imunoadsorção Enzimática
/
Técnicas Imunoenzimáticas
/
Anticorpos Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article