RNA synthesis by villus and crypt cell nuclei of rat intestinal mucosa.
Biochim Biophys Acta
; 739(3): 334-43, 1983 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-6830810
ABSTRACT
Rat intestinal mucosa was separated by eversion and vibration to provide a sequence of fractions from predominantly villus cells to predominantly crypt cells. The proportions of these cell types in each fraction were computed from the concentrations of alkaline phosphatase (villus cells) and thymidine kinase (crypt cells) in each population. The isolated mucosal fractions varied from about 90% villus cells to 90% crypt cells. Following injection of the rats with [3H]thymidine, the nuclei were isolated from each mucosal cell fraction and the amount of radioactivity incorporated into DNA was measured as an index of crypt cell abundance. The isolated nuclei were also incubated with ribonucleoside triphosphates and the amount of RNA synthesized was measured. Nuclei labeled with [3H]thymidine were found only in fractions rich in crypt cells, whereas capacity for RNA synthesis remained very active in mucosal fractions consisting predominantly of villus cells. It is concluded that non-dividing villus cells continue to make RNA.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Núcleo Celular
/
Mucosa Intestinal
Limite:
Animals
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article