Changes in physical properties of vacuolar membrane during transformation of protein bodies into vacuoles in germinating pumpkin seeds.

ABSTRACT

Changes in membrane molecular dynamics associated with the transformation of protein body membranes into vacuolar membranes during pumpkin seed germination, were monitored by EPR-spin probe technique. Using highly purified membrane preparations as well as 5-SASL and 16-SASL spin labels, parameters like general membrane lipid fluidity, order parameter, semicone angle, rotational correlation times tau 2B and tau 2C, ratio of immobilized to mobile lipids were determined and the activation energy for rotational diffusion of 16-SASL was calculated. Analysis of these parameters at different temperatures indicated a more rigid nature of protein body membrane comparing to vacuolar membrane, as a result of a more restricted motional freedom of lipids. These differences are discussed in terms of protein composition and various functional specialization of both types of membranes.